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In an additional experiment, follicles were incubated with 5 g/ml of a neutralizing goat antimouse VEGF antibody ( n = 7

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The testes of these animals were reduced in size and although epididymides were overall nearly allometric to the reduced body weight, the distal regions of the duct, vas deferens, seminal vesicles, and prostate were vestigial

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Cells were first gently lysed with the cytoplasmic extraction buffer to release cytoplasmic components, and the supernatant was collected after centrifugation as the cytoplasmic protein fraction