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To prevent reversion of D1467G to wt, we included two mutations at the nucleotide level still encoding a glycine (A4741G C4742G)

When glutathione is depleted, these detoxification pathways become impaired, leading to toxic buildup that can worsen liver damage

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[18] [19] [20] In the United States, the Food and Drug Administration (FDA) has advised that over-the-counter PPIs, such as Prilosec OTC, should be used no more than three 14-day treatment courses over one year