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To evaluate the healing efficacy, a comprehensive experiment was conducted on diabetic wounds treated with various agents, including: i) PBS as negative control (NC group), ii) ROS-responsive hydrogel matrix (G group), iii) monomer copper peptides M-CuP solution (M-CuP group), iv) dimeric copper peptides D-CuP solution (D-CuP group), v) ROS-responsive hydrogel with monomer copper peptides M-CuP (G/M-CuP group), and vi) ROS-responsive hydrogel with dimeric copper peptides (G/D-CuP group)

The 100 L serum of every sample was positioned in a 96-well plate and mixed by 300 L of precooled extract solution (methanol: acetonitrile, 2:1, v/v)

It acts through melanocortin pathways involved in sexual arousal, not through the LH-testosterone production axis

Often used in GHK-Cu peptide injection protocols that target wrinkles and skin laxity

Published in-vitro work commonly uses GHK-Cu in the range of 10 nM to 10 M, with 1 M as a frequently reported working concentration