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After removal of DTT by a G-25 Sephadex column, the titration of the reduced cysteines was done using 5,5-dithiobis-(2-nitrobenzoic acid) (DTNB)

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demonstrated that BPC-157 activates the FAK-paxillin pathway in tendon fibroblasts, directly stimulating collagen synthesis at rates 4060% above control groups