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However, a key methodological consideration for reproducible pharmacological screening is the need to optimize and standardize ethanol concentration and exposure duration to robustly induce steatosis while minimizing systemic toxicity and mortality

Environmental stresses, pollution, poor diets, and ageing all contribute to a reduction in the body's natural glutathione levels

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Then transfection was performed on cells using Lipofectamine 3000 (Invitrogen) according to the manufacturers instructions