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Hsp70-targeting and size-tunable nanoparticles combine with PD-1 checkpoint blockade to treat glioma

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HCT116 Flp-in cells (seeded in 10 cm plates, 30% confluency) were co-transfected with 9 g of donor plasmid and 3 g of sgRNA-Cas9 plasmid, and 24 l Turbofect (Thermo Scientific, R0533) in 2 ml Opti-MEM (Thermo Scientific, 11058-021)

All chemicals were used without further purification or modification

This can be particularly beneficial in preventing oxidative damage in organs like the liver and brain, which are often susceptible to the harmful effects of free radicals