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6), 25% concentrated WRNE media (cWRNE) consisting of advanced DMEM/F12 supplemented with penicillin/streptomycin (1% v/v), GlutaMAX (1% v/v), 25% (v/v) L-WRN conditioned medium 61 concentrated using 10 kDa Amicon Ultra filter unit (Merck, #UFC901008), murine EGF (50 ng/mL), 1X N2 supplement, 1X B27 supplement, N-acetyl-L cysteine (1 mM

Once you mix it with liquid, keep it in the fridge and use it up within 24 weeks

Since an exogenous antioxidant exhibits anticancer effect by both antioxidant (chemopreventive) and prooxidant (chemotherapeutic) mechanisms, the dose is a critical factor

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PDHK1 inhibition not only suppresses glycolysis but also promotes mitochondrial oxidation metabolism and reactive oxygen species (ROS) production