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Quantitative real-time PCR was performed using TB Green Premix Ex TaqTM (Takara, Osaka, Japan) on a CFX96 Real-Time PCR Detection System (Bio-rad, USA)

Glutathione helps fade dark spots and pigmentation, revealing a natural glow after just a few sessions

Amplification products (50 ng) were amplified using SYBR Green PCR Master Mix or TagMan gene expression assay (Applied Biosytems, Foster city, CA) on an Applied Biosystems 7500 Prism Sequence Detection System