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After 10 min, uptake was terminated by two PBS-CM washes followed by addition of lysis buffer (1% sodium dodecyl sulfate / 0.1 M NaOH)

Subsequent cell- and animal- based assays confirmed that Al 2 O 3 NPs caused mitochondria-dependent apoptosis and oxidative stress either in vitro or in vivo, which were consistent with the trends of gene regulation