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doi: 10.1038/s41389-017-0025-3

doi: 10.1038/NCPCARDIO0938 87 Freire JorgeP.WieringaN.de FeliceE.van der HorstI

Line thickness indicates confidence of protein interaction

Plasmid constructs FLAG-UBR5 (#37188), HA-GSK3 (#14753), HA-GSK3-S9A (#14754), and HA-GSK3-K85A expression plasmids were from Addgene (USA)

The analytical technique in that study (liquid chromatographyisotopic dilution mass spectrometry) more closely resembles the LCMS/MS assay we used than does the HPLC technique used by Borgstrm and colleagues in their 1986 study [4]