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Since the concentration of 4HNE needed for half maximal adduct formation is similar to that needed for the binding events observed via PAGE, we asked whether the slower migrating species observed via native PAGE represented a covalent 4HNE protein adduct or alternatively a traditional non-covalent protein-ligand interaction

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Images were captured using a Leica TCS SP8 confocal microscope and acquired using Leica Application Suite - Advanced Fluorescence (LAS AF) 2.6.17314 software (Leica Microsystems, Germany) and representative images are shown in (Extended Data Fig

Perturbations in the resulting lists were grouped into 11 classes: Biotic stress (Bs), Chemicals (Che), Germination (Ge), Light (Li), N-starvation (-N), Fe-deficiency (-Fe), Salt stress (Sa), Hypoxia (Hy), Drought (Dr), Heat (He), and Cold (Co)