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Cells were harvested using 110 L ice cold lysis buffer containing 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM MgCl 2 , 1% (v/v) Triton X-100 and 10% (v/v) of protease and phosphatase inhibitor cocktail (Roche)

While the assumption of no intercurrent events is often not entirely realistic, this estimate is the closest we can currently get to an estimate of the true pharmacological effect of the drug and is referred to as the trial product estimate

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