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Freezing (-20C / -4F): A Double-Edged Sword Now, this is where it gets interesting

10.1038/srep43662 109

Due to HPG axis disruption, phthalates alter the levels of GnRH by interacting with genes of G protein-coupled receptors [GPCRs] on pituitary cells and disrupt FSH and LH ratio by interfering with their receptors on Leydig cells, which consequently disrupt the normal activity of steroidogenic enzymes and steroid hormones (142)

One plausible explanation is that fatty acids of various carbon chain lengths may regulate the conformational changes and transcriptional activities of PPARs in different or even opposing manners [26]

Sequential versus simultaneous use of chemotherapy and gonadotropin-releasing hormone agonist (GnRHa) among estrogen receptor (ER)-positive premenopausal breast cancer patients: effects on ovarian function, disease-free survival, and overall survival