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Gene Expression Analysis Total RNA was extracted from 100 mg leaf, stem and root of 3-week-old seedlings or flower and silique of mature plants with TRIzol reagent and then treated with gDNA Eraser to remove DNA contamination

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Common (Usually Transient) Side Effects Water retention or puffiness Carpal tunnel-like symptoms Joint stiffness (often temporary) Mild insulin resistance if overdosed How We Monitor Baseline and follow-up IGF-1 levels Body composition changes Symptom tracking and dose titration Our goal is to maintain IGF-1 in the high-normal physiologic range , not to create supraphysiologic levels

Individuals who have difficulty absorbing nutrients or who follow strict plant-based diets may have a higher risk of developing the deficiency