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doi: 10.1016/j.bbrc.2022.01.106 201 MuriJ.KopfM

Despite these conceptual advances, current microbiome profiling methodsoften based on 16S rRNA sequencingprovide limited functional resolution, and only a subset of studies incorporates shotgun metagenomics or metabolomics to directly link microbial shifts to host pathways, leaving important mechanistic questions unresolved (7, 8)