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History Scientific interest in NNMT increased substantially during the early 2000s as researchers discovered its involvement in metabolic regulation, cellular methylation processes, and adipose tissue function

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AMPK directly phosphorylates both the fission protein MFF and the fusion and ER-mitochondria tethering protein MFN2, thereby acting as a central energy-sensing regulator that adjusts mitochondrial morphology to meet metabolic demand

Once anesthetized, the right knee joints of the animals were shaved, sterilized, and subjected to an intra-articular injection of collagenase type II

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