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Alternatively, prior to nuclear extractions, U937 cells were incubated with 10 ng ml 1 of TNF- alone or in addition to various doses of glutathione ethyl-ester (GSH-OEt, Sigma)

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The fusion of cisplatin with GSH, cysteine (L(+)-Cysteine), and other proteins leads to the depletion of antioxidant reserves in the cytoplasm and causes cellular oxidative stress, which is the major mode of action of cisplatin toxicity

Insert the moistened Suppository deep into the rectum using fingers