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Even if youre purifying the same protein over and over, it is probably still a good idea to clean and regenerate your column about every five purifications, or earlier if you notice that the binding capacity of your beads is getting worse

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GSH has been shown to exert an anti-influenza activity in vitro and in vivo, and depletion of GSH may have a fundamental role in COVID-19 and IAV infection susceptibility and disease severity 144,145

You remember, I'm older than these two guys

Life Sci, 2016