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Basal cell cycle distribution has been implicated in the radio response, with cells in the S phase and G0/G1 phases being more radioresistant, and cells in the G2/M phase being the most sensitive to radiation exposure ( Metformin treatment significantly reduced the proportion of radiosensitive HCT116 cells in the G0/G1 phase at 48 h post treatment, when compared to vehicle control ( Figure 2A )