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Sustained drug treatments suppress tumor angiogenic activity, leading to tumor-associated hypoxia, which promotes the selection of resistant cell clones adapted to oxygen and nutrient deprivation, thereby limiting drug efficacy [48]

Although emulsion droplet vitrification has emerged as a promising cryopreservation method, the upper sample size is limited by the nozzle of the inkjet head, which makes it inapplicable for mammalian oocytes, organoids and spheroids [116]

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