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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog TNFa

Berk M, Williams LJ, Jacka FN, ONeil A, Pasco JA, Moylan S, Allen NB, Stuart AL, Hayley AC, Byrne ML, Maes M (2013) So depression is an inflammatory disease, but where does the inflammation come from

The mice also received intraperitoneal injections of 0.5 g Bordetella pertussis toxin (Sigma-Aldrich, MO, USA) suspended in 0.1 ml PBS

doi:10.1073/pnas.94.5.1967 19

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