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GSH concentrations were calculated as differences between peak areas corresponding to untreated and N-ethylmaleimide-treated aliquots of the sample

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The mechanisms underlying abnormal wound healing in diabetes are complex and diverse, primarily manifested in hyperglycemia-induced oxidative stress, inflammatory responses, and significant impairment of cellular functions

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To confirm this, an affinity-purified polyclonal antibody was raised against recombinant Gpa-GSS17 and shown to be specific for this protein (Fig 2C)