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Membranes were blocked as earlier in 5% Milk/TBS-T then probed overnight with mouse anti-Catalase primary antibody (Sigma #C0979, diluted 1:1000 in 5% milk/TBS-T) at 4 C then rinsed in TBS-T buffer

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A mechanism of cell-mediated immune defence whereby an effector cell of the immune system actively lyses a target cell, whose membrane-surface antigens have been bound by specific antibodies