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Hyperglycemia resulted in decreased E

doi: 10.1371/journal.pone.0029968 108 LaCrossN

Immunoprecipitation and co-immunoprecipitation (Co-IP) Protein was extracted from cells using lysis buffer (HEPES 50 M, NaCl 150 M, EDTA 1 mM, 1% Triton, 10% glycerol) supplemented with protease inhibitor cocktail, centrifuged at 4 C, 10000 g for 30 min

The REIMAGINE 2 trial ran for 68 weeks and involved a total of 2,728 participants with type 2 diabetes that was not adequately controlled by metformin