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Following treatment with 0.5 mg/mL LPC, an initial increase in GFAP+ expression is observed over a 72-h period when compared to that of untreated slices, but with further time in culture, no difference in GFAP+ expression is seen between untreated and LPC-treated slices

To assess cell death and proliferation, 7AAD or Ki-67 (BioLegend, 420404 and 652409) staining was performed respectively

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Degenerative nucleus pulposus cells derived exosomes promoted cartilage endplate cells apoptosis and aggravated intervertebral disc degeneration

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