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Samples (20 l) were mixed with 5 l of 5x loading buffer (0.3 M Tris-HCl pH = 6.8, 25% -mercaptoethanol, 50% glycerol, 10% SDS, 1% bromophenol blue), heated to 95 C for 5 min, and 15 l of samples were applied to the gel together with 5 l of Thermo Scientific PageRuler Unstained Broad Range Protein Ladder

STING directly recruits WIPI2 to STING-positive vesicles and competes with PI3P (canonical bulk autophagy) for binding to WIPI2, adding another layer of complexity to the feedback mechanism between autophagy and cGAS-STING signaling (158) ( Figure 4 )

Moreover, in order to further reduce burden, as discussed in more detail in prior sections of this final rule with comment period, we are delaying enforcement of our requirements to encode the new data elements in the MRF

Always use a new sterile syringe and needle for each injection