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Quantitative real-time PCR(RT-PCR) was performed on a ViiATM 7 RT-PCR system (Applied Biosystems Inc.) with SYBR-green ChamQTM SYBR Color qPCR Master Mix (Vazyme)

On examination, there was diffuse hyperpigmentation predominantly seen over the face, bilateral forearms, lower legs, palms and soles [Figure 1]

It is associated with metabolic disorders such as weight loss, hypermetabolism and hyperlipidemia (Dupuis et al., 2011)

Cycle Length: Typically 812 weeks

CrossRef Comparing bone and muscle parameters in community-dwelling older adults with obesity, with or without type 2 diabetes mellitus Costas Glavas, Jakub Mesinovic, Peter R