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mellonella were recollected and homogenized in a plastic pestle homogenizer in ice-cold extraction buffer (pH 7.5) containing 250 mm sucrose, 50 mm Tris-HCl, 1 mm EDTA, and 1 mm DTT at a 1:4 w/v ratio, which was then centrifuged at 12,000 rpm for 30 min at 4C