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The reaction mixture for each well consisted of 5 L of master mix, 0.5 L of each forward and reverse primer (10 M) and 1 L of 1 in 10 dilution of cDNA

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[10] In the absence of coenzyme B12, tetrahydrofolate cannot be regenerated from its inactive storage form, 5-methyl tetrahydrofolate, resulting in functional folate deficiency