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Gene ontology (GO) classification analysis revealed that differentially expressed genes were predominantly associated with immune system processes, responses to stimuli, and metabolic regulation at the biological process level, while intracellular and protein-containing complexes were enriched at the cellular component level, and binding- and catalytic-related functions were dominant at the molecular function level (Fig

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The cell debris was precipitated by centrifugation (Thermo Scientific Sorvall LYNX 6000 centrifuge) at 30,000 g for 60 min

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