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Reaction mixtures were prepared in 100 mM Britton and Robinson buffer at different pH values (2.0, 3.0, 4.0, 5.0, 6.0, 7.0, 8.0 and 9.0) containing ABTS (1 mM), DMP (5 mM), guaiacol (50 mM), sinapic acid (0.35 mM) or p -coumaric acid (0.1 mM) in each case

doi: 10.1373/49.3.515

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Indeed, due to the vitreal-aqueous gradient, changes in the retinal secretome may reflect alterations in composition of AH, which is increasingly being used for proteomic and metabolomic analysis of retinal diseases, including glaucoma 9,13,14,15