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doi: 10.1111/j.0301-5661.2004.00148.x

Reintroduce normal activities through controlled progression rather than sudden full load

On the 31st day, animals in all groups were anesthetized with thiopental (70 mg/kg) by intraperitoneal injection and the livers were isolated, frozen in liquid nitrogen, and stored at 80 C for further biochemical assays