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88 PopugaevaE.PchitskayaE.SpeshilovaA.AlexandrovS.ZhangH.VlasovaO.et al (2015)

Baillieres Clin Haematol (1989) 2(2):195256

Tocris), TGF (100 ng ml 1 ) and TRULI (Axon) was added to our previously published mouse hepatoblast medium (MM 29 ) with minor modifications: AdDMEM/F12 (Invitrogen) supplemented with 1% HEPES, 1% GlutaMAX, 1% penicillin/streptomycin, 1 B27 without retinoic acid, 1.25 mM N -acetylcysteine, 10 nM gastrin, 50 ng ml 1 hEGF, 15% RSPO1 conditioned medium, 100 ng ml 1 FGF10, 100 ng ml 1 FGF7, 50 ng ml 1 HGF, 10 mM nicotinamide, 2 M A83-01, 3 M CHIR99021, 10 M Y-27632 and 0.5 nM Wnt surrogate Fc fusion protein