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SuperScript IV reverse transcriptase (Invitrogen) was used for cDNA synthesis and for transcript sequencing reactions, which used a 2:1 ratio of dNTPs to ddNTPs

Plasma trimethylamine N-oxide and its precursors: population epidemiology, parentchild concordance, and associations with reported dietary intake in 11- to 12-year-old children and their parents

The reduction of BDNF levels negatively impacts the signaling pathways that facilitate LTP, as BDNF is known to enhance NMDA receptor activity and promote the incorporation of AMPA receptors into the postsynaptic membrane (Peregud et al