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Likewise, the GSH-CDs synthesized with a 1:1 ratio possessed the best GP X -like activity according to the faster decrease in OD 340

Step 4: Evaluate Mechanistic Compatibility Next determine whether the compounds work together or compete

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Next, Cys63 nucleophilically attacks the sulfide of Cys58, releasing a GS-anion, which, in turn, picks up a solvent proton and is released from the enzyme, thereby creating the second GSH