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Thus, while the molecular details of adrenal mitochondrial steroid biosynthesis are well understood, as described below, the evolution of this system remains an important and poorly understood area of research (40)

First research on biomarkers focused on urinary tubular enzymes including proximal renal tubular epithelial antigen (HRTE-1), alpha-Glutathione S-transferase (alpha-GST), pi-Glutathione S-transferase (pi-GST), gamma-Glutamyltranspeptidase (gamma-GT), alanine aminopatidase (AAP), lactate dehydrogenase (LDH), N-acetyl-beta-glucosaminidase (NAG), alkaline phosphatase (ALP)

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Anion exchange elutions were polished by gel filtration using a Superose 6 Hiload 16/600 column (Cytiva) equilibrated in storage buffer (25 mM HEPES, pH 7.5, 500 mM NaCl, 0.5 mM TCEP, 5% glycerol)