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Prior to Reconstitution: Keep the lyophilized vial inside a dark, moisture-free environment

This creates a cystine/cysteine redox cycle across the membrane and creates a reducing extracellular environment that supports cancer cell growth and survival ( Cell Death Ferroptosis is an iron-dependent cell death which is accompanied by iron accumulation and lipid peroxidation

Given that mitochondrial dysfunction is often associated with oxidative stress, we also measured ROS levels using the CM-H 2 DCFDA assay

Then 10 6 cells of each of the chosen cell lines were seeded in standard culture plates of 96 wells and kept in an incubator with atmospheric conditions of 95% humidity and 5% carbon dioxide

Turn the tube so the cap faces downward and gently tap to move the cream to the bottom