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Experimental animals were housed and handled in accordance with protocols approved by the Committee on the Use of Live Animals in Teaching and Research of Shenzhen University (China)

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Nevertheless, future research should focus on examining temporal relationships between HPA axis function and immune-inflammatory or nitrosative stress pathways through biomarkers like TNF-, IL-10, TGF-, inducible NO synthase, NO production, protein nitrosylation, and bacterial translocation in ME/CFS patients to better understand their roles [79]

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