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Briefly, to separate epidermis from dermis, whole skin from mice at postnatal day (P)0-P3 were incubated in a solution of 5 mg/ml Dispase II (#D4693, Sigma-Aldrich, USA) diluted in RPMI medium (Gibco, USA) supplemented with 10% fetal calf serum (FCS, #S0615, Sigma-Aldrich, USA), penicillin (100 U/ml), streptomycin (100 g/ml), 100 nM sodium pyruvate, and 10 mM non-essential amino acids (all Gibco, USA) at 4 C overnight

In rodent models, deferoxamine given before or right after surgery preserved cognition (136)

Improved reversed phase liquid chromatographic method with pulsed electrochemical detection for tobramycin in bulk and pharmaceutical formulation

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Polyphenols and human health: the role of bioavailability