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For purification, harvested cells were first re-suspended in chilled lysis buffer (20 mM Tris [pH 7.9], 0.5 M NaCl, 5 mM imidazole, 10% glycerol, 1 mM DTT, 1 mM PMSF) and the lysis mixture was sonicated on ice

This is why people with stomach surgery, autoimmune gastritis, or long-term PPI use can develop B12 deficiency despite adequate dietary intake

This effect also starts to explain why drinking beverages other than water can be so satisfying, even when they leave you feeling thirsty later: the dopamine spike that comes from drinking liquids when animals are thirsty doesnt depend on what kind of liquid theyre drinking, even though not all liquids are equally hydrating

(1995) Targeted disruption of the 3-adrenergic receptor gene