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Glutathione is an important water-soluble antioxidant that plays a role in supporting muscles from free radicals.**

As reviewed by Semple et al

Equine behavioral enrichment toys as tools for non-invasive recovery of viral and host DNA

The homogenate was centrifuged at 13,000 g for 20 min at 4 C, and the supernatant was used for antioxidant enzyme assays

For example, proinflammatory cytokines such as IL-1 have been shown to impair neurotransmitter homeostasis (e.g., decreased serotonin levels and enhanced glutamatergic excitotoxicity), induce oxidative stress (OS) and neuronal pyroptosis, and lead to mitochondrial dysfunction and synaptic protein lossultimately fostering a bidirectional relationship between epilepsy and depression (Zhu et al., 2006