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TUNEL staining For cell death analysis, TUNEL staining of cardiac tissue section was performed using commercially available kit as per manufacturers protocol

Many of these molecules can activate receptors on the vagus nerve or cross the BBB to act in the brain

This work was supported by Sichuan Natural Science Foundation (Number: 2025ZNSFSC1801), Mianyang Central Hospital Talent Introduction Research Project (Number: 2025RCYJ-003), NHC Key Laboratory of Nuclear Technology Medical Transformation (Mianyang Central Hospital) (Number: 2024HYX020), Mianyang Central Hospital Incubation Subjects (Number: 2024FH006), National Natural Science Foundation of China (Number: 82474220), Sichuan Provincial Administration of Traditional Chinese Medicine (Number: 25MSZX305), and Mianyang Science and Technology Bureau (Mianyang Science and Technology Special Project) (Numbers: 2023ZYDF073 and 2025ZYDF019)

It can also be provided through facials with a facial cream

However, the paucity of robust cosmetic trials in this setting reflects the generalized low representation of Hispanic and Latino individuals in clinical studies of other skin conditions