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Cell viability was determined using the CellTiter-Glo Luminescent Cell Viability Assay (Promega, USA), on a Spark multimode microplate reader (TECAN, Switzerland) after 5 days

administration can be, but is not limited to, parenteral, subcutaneous, intravenous, intramuscular, intraperitoneal, modes of administration of the composition of the present disclosure can be, but are not limited to, sublingual, injectable (including short-acting, depot, implant and pellet forms injected subcutaneously or intramuscularly), or by use of vaginal creams, suppositories, pessaries, vaginal rings, rectal suppositories, intrauterine devices, and transdermal forms such as patches and creams

Indeed, HA-tagged NEDD8 or ubiquitin, but not SUMO2 or SUMO3, immunoprecipitated modified high-molecular weight IL-1 when expressed in 293T cells (Supplementary Fig

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But whole blood levels dont move as much or even in any significant amount in response to supplementation