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and Kaplowitz, N

Urinary-derived extracellular vesicles reveal a distinct microRNA signature associated with the development and progression of Fabry nephropathy

The indicator cells were cultivated overnight with methionine (in the sulfur auxotroph) and GSH (in the gsh1 strain) in the pre-cultures and starved without these sources for 5-6 hours before re-inoculating at an initial OD 600 nm of 0.15 in media containing 100 M of GSH (for the sulfur auxotrophic strain) and no GSH for the glutathione deficient strain

Singer P, Berger MM, Van den Berghe G, Biolo G, Calder P, Forbes A et al

doi: 10.1128/mBio.02123-20 40 BurrackL