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Bescond A, Augier T, Chareyre C, Garcon D, Hornebeck W, and Charpiot P (1999)

1 Introduction Viral vectors are modified viruses that deliver therapeutic genetic material to target cells and are an emerging platform for gene therapy ( A prototype strain of coxsackievirus A21 (CVA21), a non-enveloped enterovirus in the Picornaviridae family, is undergoing clinical evaluation as an oncolytic virus immunotherapy application for the treatment of several types of cancers ( Based on reports that enteroviruses interact with intracellular antioxidant glutathione (GSH) during capsid assembly in infected cells ( The objective of this work was to establish a robust and scalable purification method of CVA21 with high infectivity yield and purity that can accommodate variable cell culture conditions

Moderate to high throughput in vitro binding kinetics for drug discovery

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