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The resulting trimmed and de-duplicated reads were then aligned to the Rattus norvegicus Rnor6.0 reference genome, available on ENSEMBL, using STAR aligner v.2.5.2b4 25 , producing BAM files

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Absorption matters: age, meds, low intrinsic factor, or restricted diets raise deficiency riskconsistent, well-absorbed forms help maintain healthy levels