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Avidin:biotin interactions are so strong that elution of biotin-tagged proteins from avidin-conjugated resins usually requires denaturing conditions

Its also the single most protective antioxidant produced by the body and is therefore the most important for protecting cells against oxidative stress from dietary and environmental free radicals as well as those resulting from normal metabolic processes

The pH values of the samples were determined using a Metrohm 6.0204.100 combined pH glass electrode, calibrated by aqueous NBS standard buffer solutions