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Drops (2 L) consisting of a 1:1 ratio of C20A mutant OsDHAR protein (21 mg/mL in 20 mM Tris-HCl, pH 8.0 and 150 mM NaCl) and crystallization solution (100 mM Bis-Tris HCl, pH 6.16.8, 28% (w/v) PEG 3350 and 10 mM CaCl 2 ) were equilibrated against 0.5 mL of the same solution

Biocompatibility was validated with CCK-8 assays, hemolysis assays, hematoxylin-eosin staining, and blood biochemical evaluations

Peptide samples were separated with a 60 min linear gradient from 5% to 40% ACN in 15 mM ammonium hydroxide at a flow rate of 0.4 mL/min

The process is quick and carried out in a clinical setting, ensuring comfort and safety.

BMAL1 collaborates with CLOCK to directly promote DNA double-strand break repair and tumor chemoresistance