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Subsequently, the slides were permeabilized with PBS buffer containing 0.2% Triton X-100 for 15 min and blocked with PBS buffer containing 5% BSA for 1 h

Pair these with selective NO/ONOO probes applied under kinetic and scavenger/enzyme controls so they define flux and location rather than a pooled ROS signal (Zhou et al

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Without the human safety data and a lack of FDA approval, sourcing peptides from an unregulated gray-market source introduces real risks of contamination, inaccurate dosing, and unknown ingredients