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For fluorescence labelling of the intestinal lumen age staged animals were transferred to plates containing 10.000 MW Alexa Fluor 546 labelled dextran (Molecular Probes, Eugene, OR, D-22911) in the presence of OP50 bacteria and incubated at 18 C

Diabetic patients with peripheral neuropathy have significantly worse everyday functioning and well-being, according to a pilot study assessing the effect of DPN on quality of life (98)